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Chapters
- 00:00Introduction
- 01:31Preparing the micropatterned membrane
- 07:01Assembly of the stretch chambers
- 15:56Seeding cells on the membrane
- 22:02Cyclic stretch
- 23:53Conclusion
It is widely understood that mechanical forces in the body can influence cell differentiation and proliferation. Here we present a video protocol demonstrating the use of a custom-built bioreactor for delivering uniaxial cyclic tensile strain to stem cells cultured on flexible micropatterned substrates.
Tags
Mechanical StimulationStem CellsCyclic Uniaxial StrainBone RemodelingMuscular HypertrophySmooth Muscle Cell PlasticityIn Vitro MethodAdherent CellsElastic MembranesBioreactorMotorized Cam-rotor SystemStep-by-step Video ProtocolStretch ChamberSilicone MembraneMicropatterned TopographyStrain DirectionSterilizing ChambersSeeding CellsAdjusting Mechanical ParametersHuman Mesenchymal Stem Cells
