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JoVE Journal Biology
Loading Drosophila Nerve Terminals with Calcium Indicators
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Loading Drosophila Nerve Terminals with Calcium Indicators

Article DOI: 10.3791/250-v • 15:16 min • July 30th, 2007
July 30th, 2007 •
1, 1
1Department of Physiology, University of Texas Health Science Center at San Antonio (UTHSCSA)

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Calcium is a ubiquitous messenger in the nervous system, essential for triggering neurotransmitter release and changes in synaptic strength. Here we demonstrate a technique for loading Ca2+-indicators into Drosophila nerve terminals. We also demonstrate fabrication of the required apparatus and emphasize points critical for the technique's success.

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Drosophila Nerve Terminals Calcium Indicators Neurotransmitter Release Synaptic Plasticity Learning And Memory Genetically Malleable Physiologically Accessible Genetically-encoded Fluorescent Indicators Ca2+ Changes Synthetic Fluorescent Indicators Dextran-conjugated Synthetic Ca2+ Indicators Live Nerve Terminals Drosophila Larvae Static Electricity Discharges Extended Loading Periods Axon Survival Low Affinity Dextran-conjugated Ca2+-indicators Fluo-4 Rhod
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