Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Crystallization of Membrane Proteins in Lipidic…
JoVE Journal
Biology

This content is Open Access.

JoVE Journal Biology
Crystallization of Membrane Proteins in Lipidic Mesophases
Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass
Journal (Biology)
Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass
Maintaining Laboratory Cultures of Gryllus bimaculatus, a Versatile Orthopteran Model for Insect Agriculture and Invertebrate Physiology
Journal (Biology)
Maintaining Laboratory Cultures of Gryllus bimaculatus, a Versatile Orthopteran Model for Insect Agriculture and Invertebrate Physiology
 
Click here for the English version

Crystallization of Membrane Proteins in Lipidic Mesophases

Article DOI: 10.3791/2501-v • 11:53 min • March 28th, 2011
March 28th, 2011 •
1, 1
1Molecular Biology, The Scripps Research Institute

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

The protocols describe the essential steps for obtaining diffraction quality crystals of a membrane protein starting from reconstitution of the protein in a lipidic cubic phase (LCP), finding initial conditions with LCP-FRAP pre-crystallization assays, setting up LCP crystallization trials and harvesting crystals.

Tags

Crystallization Membrane Proteins Lipidic Mesophases Signal Transduction Transport Energy Transformations Malfunctions Diseases Structural Understanding Functional Understanding Solubilization Diffraction Quality Crystals In Meso Crystallization Native-like Membrane Environment Solvent Content Ordering Traditional Crystallization Detergent Solutions Lipid Phase Behavior Viscous Mesophase Materials LCP (Lipid Cubic Phase) Syringe Mixer Reconstitution Lipid Bilayer Assay Plate Crystallization Plate
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter