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Dissection of Adult Mouse Utricle and Adenovirus-mediated Supporting-cell Infection
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Dissection of Adult Mouse Utricle and Adenovirus-mediated Supporting-cell Infection
DOI:

11:13 min

March 28, 2012

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Chapters

  • 00:05Title
  • 02:51Utricle Dissection and Culture
  • 06:26Adenovirus Infection of Supporting Cells
  • 07:39Utricle Fixation, Otoconia Removal, and Immunochemistry
  • 09:06Results: Adenovirus-mediated Infection of Supporting Cells
  • 10:19Conclusion

Summary

Automatic Translation

Mechanosensory hair cells are the receptor cells of the inner ear. The best-characterized in vitro model system of mature mammalian hair cells utilizes organ cultures of utricles from adult mice. We present the dissection of the adult mouse utricle, and we demonstrate adenovirus-mediated infection of supporting cells in cultured utricles.

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