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A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain
DOI:

09:49 min

March 07, 2012

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Chapters

  • 00:05Title
  • 01:40Surgery Preparation and Animal Mounting
  • 04:02The Generation of a Polished and Reinforced Thinned-skull Window
  • 07:14Imaging Preparation
  • 08:20Representative Results for Long-term Imaging and optical Manipulation of the Mouse Cortex
  • 09:30Conclusion

Summary

Automatic Translation

We present a method to form an imaging window in the mouse skull that spans millimeters and is stable for months without inflammation of the brain. This method is well suited for longitudinal studies of blood flow, cellular dynamics, and cell/vascular structure using two-photon microscopy.

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