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In ovo Electroporation in Chick Midbrain for Studying Gene Function in Dopaminergic Neuron Development
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JoVE Journal Neuroscience
In ovo Electroporation in Chick Midbrain for Studying Gene Function in Dopaminergic Neuron Development

In ovo Electroporation in Chick Midbrain for Studying Gene Function in Dopaminergic Neuron Development

DOI:
10.3791/4017-v

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08:57 min

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August 03, 2012

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, ,
1Northwestern University Feinberg School of Medicine,Children’s Hospital of Chicago Research Center, 2Departments of Pediatrics, Neurology and Physiology,Northwestern University Feinberg School of Medicine

Chapters

  • 00:05Title
  • 01:34Egg Preparation
  • 02:42Glass Injection Needle Preparation
  • 03:25Midbrain Injection
  • 04:38Electroporation
  • 05:39Incubate and Harvest
  • 06:47Results: Embryonic Chick Midbrain In ovo Electroporation Assay
  • 08:13Conclusion

Summary

Automatic Translation

Automatic Translation

To assess the function and the regulation of genes during the development of midbrain dopaminergic neurons, we describe a method that involves in ovo electroporation of plasmid DNA constructs into embryonic chick ventral midbrain dopaminergic neuron progenitors. This technique can be used to achieve efficient expression of genes of interest to study different aspects of midbrain development and dopaminergic neuron differentiation.

Tags

Ovo ElectroporationChick MidbrainGene FunctionDopaminergic Neuron DevelopmentParkinson’s DiseaseSchizophreniaDepressionDementiaNeural ProgenitorsGene Expression ProgramsExperimental ManipulationsChick Embryonic Neural TubesPCAG VectorCMV Promoter

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