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Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass
JoVE Journal
Biology
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JoVE Journal Biology
Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass
DOI:

14:29 min

May 01, 2013

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Chapters

  • 00:05Title
  • 01:10Preparation of Chromatin
  • 03:58Chromatin Immunoprecipitation (ChIP)
  • 08:38Analysis
  • 12:04Results: DNA Enrichment from Primary Lymphocytes using ChIP
  • 13:52Conclusion

Summary

Automatic Translation

We describe a robust method for chromatin immunoprecipitation using primary T cells. The method is founded on standard approaches, but uses a specific set of conditions and reagents that improve efficiency for limited a quantities of cells. Importantly, a detailed description of the data analysis phase is presented.

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