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Identification of Sleeping Beauty Transposon Insertions in Solid Tumors using Linker-mediated PCR
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Identification of Sleeping Beauty Transposon Insertions in Solid Tumors using Linker-mediated PCR

Identification of Sleeping Beauty Transposon Insertions in Solid Tumors using Linker-mediated PCR

DOI:
10.3791/50156-v

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10:34 min

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February 01, 2013

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,
1Department of Obstetrics, Gynecology & Women’s Health, Masonic Cancer Center,University of Minnesota, Minneapolis, 2Department of Genetics, Cell Biology & Development, Center for Genome Engineering,University of Minnesota, Minneapolis

Chapters

  • 00:05Title
  • 01:00Tumor Collection and DNA Isolation
  • 02:16Digesting and Ligating Linkers to the DNA
  • 05:51Removing Concatamer Transposons Using a Second Digestion
  • 06:54Amplifying the Tumor DNA
  • 09:19PCR and Sequencing Results
  • 10:08Conclusion

Summary

Automatic Translation

Automatic Translation

A method of identifying unknown drivers of carcinogenesis using an unbiased approach is described. The method uses the Sleeping Beauty transposon as a random mutagen directed to specific tissues. Genomic mapping of transposon insertions that drive tumor formation identifies novel oncogenes and tumor suppressor genes

Tags

Sleeping Beauty TransposonForward Genetic ScreenLinker-mediated PCRTumor GeneticsCancer GenomeGenetic DriversPassenger MutationsGenetic HeterogeneityCre RecombinaseTransposaseCommon Insertion Sites

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