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Generation of Aligned Functional Myocardial Tissue Through Microcontact Printing
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Bioengineering
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JoVE Journal Bioengineering
Generation of Aligned Functional Myocardial Tissue Through Microcontact Printing

Generation of Aligned Functional Myocardial Tissue Through Microcontact Printing

DOI:
10.3791/50288-v

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11:09 min

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March 19, 2013

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,
1Cardiovascular Research Center,Massachusetts General Hospital and Harvard Medical School, 2Harvard Stem Cell Institute

Chapters

  • 00:05Title
  • 01:19Microcontact Printing of Fibronectin onto PDMS Substrates
  • 02:46Maintenance of Double Transgenic Embryonic Stem Cell Lines
  • 04:37In vitro Differentiation of Double Transgenic Embryonic Stem Cell Lines and Isolation and Seeding of Cardiac Progenitors
  • 08:14Results: Highly Purified Populations of Embryonic Stem Cell Derived Cardiac Progenitors
  • 10:51Conclusion

Summary

Automatic Translation

Automatic Translation

The generation of aligned myocardial tissue is a key requirement for adapting the recent advances in stem cell biology to clinically useful purposes. Herein we describe a microcontact printing approach for the precise control of cell shape and function. Using highly purified populations of embryonic stem cell derived cardiac progenitors, we then generate anisotropic functional myocardial tissue.

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Microcontact PrintingStem Cell-derived CardiomyocytesHeart FailureExtracellular MatrixCell Alignment

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