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Three-dimensional Imaging of Nociceptive Intraepidermal Nerve Fibers in Human Skin Biopsies
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Three-dimensional Imaging of Nociceptive Intraepidermal Nerve Fibers in Human Skin Biopsies

Three-dimensional Imaging of Nociceptive Intraepidermal Nerve Fibers in Human Skin Biopsies

DOI:
10.3791/50331-v

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11:22 min

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April 29, 2013

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, , , ,
1Department of Neurology,University of Michigan , 2Department of Internal Medicine,University of Michigan

Chapters

  • 00:05Title
  • 02:06Immunohistochemistry of Skin Biopsies
  • 05:47Mounting and Confocal Microscopy of Specimens
  • 07:58Three-dimensional Visualization and Animation
  • 09:00Results: Nociceptive Intraepidermal Nerve Fibers in Painful Neuropathies
  • 10:30Conclusion

Summary

Automatic Translation

Automatic Translation

In order to study the changes of nociceptive intraepidermal nerve fibers (IENFs) in painful neuropathies (PN), we developed protocols that could directly examine three-dimensional morphological changes observed in nociceptive IENFs. Three-dimensional analysis of IENFs has the potential to evaluate the morphological changes of IENF in PN.

Tags

3D ImagingNociceptive Intraepidermal Nerve FibersSkin BiopsyPeripheral PolyneuropathyPGPTrkAConfocal MicroscopyNeuropathy

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