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Chapters
- 00:05Title
- 01:52Preparation of C12FDG, Bafilomycin A1, and Cell Culture
- 03:22Induction of Senescence and Bafilomycin A1 Treatment
- 04:38C12FDG Staining
- 05:29Flow Cytometry Analysis
- 07:20Representative Assay Results
- 08:39Conclusion
Whether senescence prevents or promotes tumorigenesis remains controversial. Since chemotherapeutical drugs can induce cancer cells to senesce, studying senescence is essential for proposing new therapies. However, the standard and broadly used β-galactosidase assay presents major drawbacks. We propose here a rapid and sensitive flow cytometry-based assay to quantify senescence.
