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Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells
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Bioengineering
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JoVE Journal Bioengineering
Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells

DOI:
10.3791/50633-v

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12:22 min

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July 22, 2013

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, , , ,
1Department of Pathology and Cell Biology,Columbia University, 2Herbert Irving Comprehensive Cancer Center,Columbia University

Chapters

  • 00:05Title
  • 02:48Growth of Cells and Preparation for Imaging
  • 03:29Mito-roGFP1 Imaging
  • 04:33MitGO-ATeam2 Imaging
  • 06:09ImageJ Analysis
  • 08:40Measuring Mitochondrial Redox State and ATP with Mito-roGFP1 and MitGO-ATeam2
  • 11:22Conclusion

Summary

Automatic Translation

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We describe the use of two ratiometric, genetically encoded biosensors, which are based on GFP, to monitor mitochondrial redox state and ATP levels at subcellular resolution in living yeast cells.

Tags

Mitochondrial Redox StateMitochondrial ATPRatiometric BiosensorsRoGFPMitGO-ATeamFRETYeast CellsEnergy MetabolismCalcium HomeostasisCellular LifespanProgrammed Cell Death

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