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Temporal Quantification of MAPK Induced Expression in Single Yeast Cells
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JoVE Journal Biology
Temporal Quantification of MAPK Induced Expression in Single Yeast Cells

Temporal Quantification of MAPK Induced Expression in Single Yeast Cells

DOI:
10.3791/50637-v

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07:59 min

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October 04, 2013

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, ,
1Department of Fundamental Microbiology,University of Lausanne

Chapters

  • 00:05Title
  • 01:25Microscopy Measurements
  • 03:20Flow Cytometry Measurements
  • 05:02Results: Representative Microscopy and Flow Cytometry Measurement Data
  • 07:26Conclusion

Summary

Automatic Translation

Automatic Translation

Two complementary methods based on flow cytometry and microscopy are presented which enable the quantification, at the single cell level, of the dynamics of gene expression induced by the activation of a MAPK pathway in yeast.

Tags

Gene ExpressionSingle CellMAPK PathwayYeastFlow CytometryMicroscopyFluorescent ReporterProtein ExpressionLive Cell Imaging

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