Journal
/
Neuroscience
/
An In-vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
An In-vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse

An In-vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse

DOI:
10.3791/50688-v

•

10:34 min

•

August 07, 2013

•

, , , ,
1Pharmacology and Toxicology,Virginia Commonwealth University, 2Physiology and Biophyics,Virginia Commonwealth University

Chapters

  • 00:05Title
  • 02:04Longitudinal Muscle/Myenteric Plexus Harvesting
  • 04:42LMMP Digestion
  • 07:18Results: An in vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse
  • 09:16Conclusion

Summary

Automatic Translation

Automatic Translation

We demonstrate a cell culture protocol for the direct study of neuronal and glial components of the enteric nervous system. A neuron/glia mixed culture on coverslips is prepared from the myenteric plexus of adult mouse providing the ability to examine individual neuron and glia function by electrophysiology, immunohistochemical, etc.

Tags

Enteric Nervous SystemMyenteric PlexusIn Vitro PreparationIsolated NeuronsEnteric GliaMouse IleumColonEnzymatic DigestionNeurite OutgrowthAction PotentialsGFAP StainingImmunohistochemistryElectrophysiologyCalcium ImagingSingle-cell PCRGenetically Modified Animals

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Studying the Integration of Adult-born Neurons

The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells

An Isolated Semi-intact Preparation of the Mouse Vestibular Sensory Epithelium for Electrophysiology and High-resolution Two-photon Microscopy

An In Vitro Adult Mouse Muscle-nerve Preparation for Studying the Firing Properties of Muscle Afferents

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions

A Simple One-step Dissection Protocol for Whole-mount Preparation of Adult Drosophila Brains

Primary Culture of Neurons Isolated from Embryonic Mouse Cerebellum

Cochlear Surface Preparation in the Adult Mouse

Isolation and Culture of Primary Neurons and Glia from Adult Rat Urinary Bladder

Read Article