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Confocal Imaging of Single Mitochondrial Superoxide Flashes in Intact Heart or In Vivo
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Confocal Imaging of Single Mitochondrial Superoxide Flashes in Intact Heart or In Vivo

Confocal Imaging of Single Mitochondrial Superoxide Flashes in Intact Heart or In Vivo

DOI:
10.3791/50818-v

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12:06 min

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November 05, 2013

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,
1Mitochondria and Metabolism Center, Department of Anesthesiology and Pain Medicine,University of Washington

Chapters

  • 00:05Title
  • 01:49Confocal Imaging of Skeletal Muscles In Vivo
  • 04:30Confocal Imaging of the Perfused Mouse Heart
  • 07:18Image Processing and Data Analysis
  • 09:20Representative Mitochondrial Superoxide Flash Imaging Results
  • 11:23Conclusion

Summary

Automatic Translation

Automatic Translation

Confocal scanning microscopy is applied for imaging single mitochondrial events in perfused heart or skeletal muscles in live animal. Real-time monitoring of single mitochondrial processes such as superoxide flashes and membrane potential fluctuations enables the evaluation of mitochondrial function in a physiologically relevant context and during pathological perturbations.

Tags

Confocal ImagingMitochondrial SuperoxideSingle Mitochondrial FlashesIntact HeartIn VivoTransgenic MiceMt-cpYFPLangendorff PerfusionReal-time ImagingMitochondrial FunctionMitochondrial DysfunctionIschemiaReperfusion

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