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Assays for the Identification of Novel Antivirals against Bluetongue Virus
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Immunology and Infection
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JoVE Journal Immunology and Infection
Assays for the Identification of Novel Antivirals against Bluetongue Virus

Assays for the Identification of Novel Antivirals against Bluetongue Virus

DOI:
10.3791/50820-v

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12:02 min

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October 11, 2013

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, ,
1Division of Infectious Diseases, Department of Medicine,University of Alabama at Birmingham, 2Molette Laboratory for Drug Discovery, Department of Chemistry and Biochemistry,Auburn University

Chapters

  • 00:05Title
  • 01:37Cytopathic Effect Based Assay Using the CellTiterGlo Kit
  • 03:31Dose Response Assay
  • 06:28Time-of-Addition Assay
  • 08:55Representative Antiviral Compound Assay Results
  • 11:34Conclusion

Summary

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Three assays, including the cytopathic effect (CPE)-based assay, dose-response assay and Time-of-Addition (ToA) assay have been developed, optimized, validated and utilized to identify novel antivirals against Bluetongue virus (BTV), as well as to determine the possible Mechanism-of-Action (MoA) for newly identified antivirals.

Tags

Antiviral AssaysBTV AntiviralsCytopathic Effect (CPE) AssayDose-response AssayMechanism Of Action (ToA) AssayAntiviral ScreeningViral LifecycleCell Culture System

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