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Stress-induced Antibiotic Susceptibility Testing on a Chip
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Bioengineering
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JoVE Journal Bioengineering
Stress-induced Antibiotic Susceptibility Testing on a Chip

Stress-induced Antibiotic Susceptibility Testing on a Chip

DOI:
10.3791/50828-v

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12:41 min

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January 08, 2014

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, , , ,
1Fraunhofer USA Center for Manufacturing Innovation, 2Division of Infectious Diseases, Department of Medicine, Brigham and Women’s Hospital,Harvard Medical School, 3Department of Mechanical Engineering,Boston University, 4Department of Biomedical Engineering,Boston University

Chapters

  • 00:05Title
  • 01:40Making the Poly(dimethylsiloxane) (PDMS) Layer
  • 02:29Assembling the Flow Cell
  • 03:41Loading the Bacterial Suspension
  • 05:08Loading the Solutions
  • 06:15Image Acquisition
  • 08:02Disinfecting the Flow Cell and Counting the Bacteria
  • 09:21Results: Representative Bacterial Susceptibility Imaging and Survival Data
  • 12:07Conclusion

Summary

Automatic Translation

Automatic Translation

We have developed a microfluidic platform for rapid antibiotic susceptibility testing. Fluid is passed at high speeds over bacteria immobilized on the bottom of a microfluidic channel. In the presence of stress and antibiotic, susceptible strains of bacteria die rapidly. However, resistant bacteria can survive these stressful conditions.

Tags

MicrofluidicsAntibiotic Susceptibility TestingStress-based TestingBacterial CulturePDMSCell ViabilityFluorescenceImage ProcessingMechanical StressEnzymesMicroarray SubstratesDrug DevelopmentBacterial Suspension Experiments

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