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Excitotoxic Stimulation of Brain Microslices as an In vitro Model of Stroke
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JoVE Journal Medicine
Excitotoxic Stimulation of Brain Microslices as an In vitro Model of Stroke

Excitotoxic Stimulation of Brain Microslices as an In vitro Model of Stroke

DOI:
10.3791/51291-v

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07:00 min

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February 04, 2014

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, , ,
1School of Biomedical Sciences and Pharmacy, Hunter Medical Research Institute,The University of Newcastle, 2School of Health and Human Sciences,Southern Cross University, 3School of Medicine and Public Health,The University of Newcastle

Chapters

  • 00:05Title
  • 01:08Preparation of Microslices
  • 02:27Equilibration of Microslices
  • 03:46Excitotoxic Stimulation
  • 05:26Results: Viability of Microslices
  • 06:41Conclusion

Summary

Automatic Translation

Automatic Translation

We have developed a brain slice model which can be used to examine molecular mechanisms involved in excitotoxicity-mediated brain injury. This technique generates viable mature brain tissue and reduces animal numbers required for experimentation, whilst keeping the neuronal circuitry, cellular interactions, and postsynaptic compartments partly intact.

Tags

Brain MicroslicesIn Vitro ModelStrokeExcitotoxicityNeuropathologyIschemic StrokeCell CultureMolecular PathwaysBrain RegionsExcitotoxic StimulationInhibitors

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