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DNBS/TNBS Colitis Models: Providing Insights Into Inflammatory Bowel Disease and Effects of Dietary Fat
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JoVE Journal Medicine
DNBS/TNBS Colitis Models: Providing Insights Into Inflammatory Bowel Disease and Effects of Dietary Fat

DNBS/TNBS Colitis Models: Providing Insights Into Inflammatory Bowel Disease and Effects of Dietary Fat

DOI:
10.3791/51297-v

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09:04 min

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February 27, 2014

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, , , , , ,
1Division of Gastroenterology,BC Children’s Hospital

Chapters

  • 00:05Title
  • 01:24Intra Rectal Dinitrobenzene Sulfonic Acid (DNBS) Administration
  • 02:52Collecting Tissues and Assessing Histological Damage in DNBS Challenged Mice
  • 04:27Myeloperoxidase (MPO) Assay of DNBS Challenged Intestinal Tissue
  • 05:25Results: Representative Effects of Dietary Oils on DNBS Colitis Severity of DNBS Challenged Intestinal Tissue
  • 08:18Conclusion

Summary

Automatic Translation

Automatic Translation

DNBS/TNBS induced colitis offers an alternative and inexpensive method to study the pathobiology of inflammatory bowel disease. The protocol discussed in this paper outlines the successful application of DNBS to induce colitis in mice and rats and allows one to thoroughly study host-mediated intestinal responses.

Tags

DNBSTNBSColitisInflammatory Bowel DiseaseDietary FatN-3 Fatty AcidsN-6 Fatty AcidsSafflower OilCanola OilFish OilWeight LossMucosal InflammationMyeloperoxidase

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