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Journal
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Immunology and Infection
/
Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry
Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry
DOI:
10.3791/51345-v
•
07:31 min
•
March 28, 2014
•
Olimpia Gamucci
,
Alice Bertero
2
,
Maria Ada Malvindi
,
Stefania Sabella
,
Pier Paolo Pompa
,
Barbara Mazzolai
,
Giuseppe Bardi
1
Center for Micro-BioRobotics @SSSA
,
Istituto Italiano di Tecnologia
,
2
Department of Biology
,
University of Pisa
,
3
Center for Biomolecular Nanotechnologies @UniLe
,
Istituto Italiano di Tecnologia
Chapters
00:05
Title
00:56
Nanoparticle Internalization in Blood Leukocytes and Purified Monocytes
02:22
Flow Cytometric Analysis
03:44
Results: Representative FITC-SiO
2
Nanoparticle Internalization Analyses
06:55
Conclusion
Summary
Automatic Translation
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Automatic Translation
Analysis of nanoparticle interaction with defined subpopulations of immune cells by flow cytometry.
Tags
Flow Cytometry
Nanoparticle Interaction
Immune Cells
FITC-SiO2 Nanoparticles
Rhodamine-SiO2 Nanoparticles
Primary Microglia
GFP-expressing Monocyte/macrophage Lineage
Cell Fluorescence
Side-scattered Light
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