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Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method
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Bioengineering
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JoVE Journal Bioengineering
Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method

DOI:
10.3791/51501-v

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07:56 min

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May 08, 2014

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, , , , , ,
1Department of Electrical and Computer Engineering,University of Minnesota, 2Department of Biomedical Engineering,University of Minnesota, 3Department of Neurology,Mayo Clinic College of Medicine, 4Department of Immunology,Mayo Clinic College of Medicine

Chapters

  • 00:05Title
  • 01:27Preparation of Vesicles
  • 02:30Formation of Spherical Supported Lipid Bilayers (SSLB)
  • 03:43Assembling the SSLB Array
  • 04:32Cholera Toxin Binding Assay
  • 05:31Typical Microwell Intensities and Array Applications
  • 07:12Conclusion

Summary

Automatic Translation

Automatic Translation

Supported lipid bilayers and natural membrane particles are convenient systems that can approximate the properties of cell membranes and be incorporated in a variety of analytical strategies. Here we demonstrate a method for preparing microarrays composed of supported lipid bilayer-coated SiO2 beads, phospholipid vesicles or natural membrane particles.

Tags

Biomembrane MicroarraySupported Lipid BilayersSpherical Supported Lipid BilayersSqueegee-based AssemblyMicrofabricated MicrowellsCholera ToxinGM1 GangliosideLipid VesiclesCellular Biomembranes

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