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Quantifying Single Microvessel Permeability in Isolated Blood-perfused Rat Lung Preparation
JoVE Journal
Biology
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JoVE Journal Biology
Quantifying Single Microvessel Permeability in Isolated Blood-perfused Rat Lung Preparation
DOI:

07:22 min

June 30, 2014

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Chapters

  • 00:05Title
  • 01:07Isolating the Rat Lung With Attached Heart
  • 02:25Blood Perfusion of the Isolated Rat Lung
  • 03:40Microvascular Infusion of the Lung
  • 06:05Vascular Permeability after Bacterial Lipopolysaccharide Treatment
  • 06:58Conclusion

Summary

Automatic Translation

The isolated blood-perfused lung preparation makes it feasible to visualize microvessel networks on the lung surface. Here we describe an approach to quantify permeability of single microvessels in isolated lungs using real time fluorescence imaging.

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