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Chapters
- 00:05Title
- 01:10Barrett’s Esophagus Cell Plating and Drug Treatment
- 03:05Immunofluorescent Staining
- 06:55Imaging on a Standard IF Microscope
- 07:42Results: IF of Immortalized BE Cells Following Treatment with an Inhibitor of Src Kinase
- 08:30Conclusion
There is a discernible need for improved information on molecular drivers of Barrett’s Esophagus. Immunofluorescent staining is a useful technique for understanding the effects of cell signaling on cell morphology. We present a simple, effective protocol for the use of immunofluorescent staining to assess therapeutic treatment in Barrett’s Esophagus cells.
