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An Immunofluorescent Method for Characterization of Barrett’s Esophagus Cells
JoVE Journal
Biology
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JoVE Journal Biology
An Immunofluorescent Method for Characterization of Barrett’s Esophagus Cells
DOI:

08:54 min

July 20, 2014

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Chapters

  • 00:05Title
  • 01:10Barrett’s Esophagus Cell Plating and Drug Treatment
  • 03:05Immunofluorescent Staining
  • 06:55Imaging on a Standard IF Microscope
  • 07:42Results: IF of Immortalized BE Cells Following Treatment with an Inhibitor of Src Kinase
  • 08:30Conclusion

Summary

Automatic Translation

There is a discernible need for improved information on molecular drivers of Barrett’s Esophagus. Immunofluorescent staining is a useful technique for understanding the effects of cell signaling on cell morphology. We present a simple, effective protocol for the use of immunofluorescent staining to assess therapeutic treatment in Barrett’s Esophagus cells.

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