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Immunology and Infection
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Isolation, Identification, and Purification of Murine Thymic Epithelial Cells
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation, Identification, and Purification of Murine Thymic Epithelial Cells

Isolation, Identification, and Purification of Murine Thymic Epithelial Cells

DOI:
10.3791/51780-v

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07:20 min

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August 08, 2014

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1Department of Laboratory Medicine & Pathology, Center for Immunology,University of Minnesota

Chapters

  • 00:05Title
  • 01:24Stromal Cell Isolation from Mouse Thymus
  • 03:20TEC Identification, Panning, and Purification
  • 04:48Results: Representative Thymic Epithelial Populations
  • 06:44Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe an efficient method for isolation, identification, and purification of mouse thymic epithelial cells (TECs). The protocol can be utilized for studies of thymus function for normal T cell development, thymus dysfunction, and T cell reconstitution.

Tags

Thymic Epithelial CellsThymusT Cell DevelopmentCell IsolationCell PurificationCell CharacterizationFlow CytometryCell DepletionCell Sorting

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