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Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting
JoVE Journal
Medicine
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JoVE Journal Medicine
Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting
DOI:

07:36 min

May 01, 2015

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Chapters

  • 00:05Title
  • 01:14Foreskin and Lymphatic Malformation Cell Suspension Preparation
  • 04:01Antibody Staining of Lymphatic Endothelial Cell (LEC) Surface Markers
  • 05:49Results: Representative Foreskin and Lymphatic Malformation LECs
  • 06:53Conclusion

Summary

Automatic Translation

The goal of this protocol is to isolate lymphatic endothelial cells lining human lymphatic malformation cyst-like vessels and foreskins using fluorescence-activated cell sorting (FACS). Subsequent cell culturing and expansion of these cells permits a new level of experimental sophistication for genetic, proteomic, functional and cell differentiation studies.

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