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Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting
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Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting

DOI:
10.3791/52691-v

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07:36 min

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May 01, 2015

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, , , , ,
1Murdoch Childrens Research Institute,The Royal Children’s Hospital, 2Department of Paediatrics, Faculty of Medicine, Dentistry and Health Sciences,The University of Melbourne, 3Department of Anatomy and Developmental Biology, Faculty of Medicine, Nursing and Health Sciences,Monash University, Clayton

Chapters

  • 00:05Title
  • 01:14Foreskin and Lymphatic Malformation Cell Suspension Preparation
  • 04:01Antibody Staining of Lymphatic Endothelial Cell (LEC) Surface Markers
  • 05:49Results: Representative Foreskin and Lymphatic Malformation LECs
  • 06:53Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to isolate lymphatic endothelial cells lining human lymphatic malformation cyst-like vessels and foreskins using fluorescence-activated cell sorting (FACS). Subsequent cell culturing and expansion of these cells permits a new level of experimental sophistication for genetic, proteomic, functional and cell differentiation studies.

Tags

Lymphatic Endothelial CellsLymphatic System DisordersPrimary LymphedemaLymphatic MalformationsLymphatic TumorsFluorescence-activated Cell SortingCD34CD31VEGFR-3PodoplaninCell IsolationCell Culture

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