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In Vitro Model of Physiological and Pathological Blood Flow with Application to Investigations of Vascular Cell Remodeling
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Bioengineering
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JoVE Journal Bioengineering
In Vitro Model of Physiological and Pathological Blood Flow with Application to Investigations of Vascular Cell Remodeling

In Vitro Model of Physiological and Pathological Blood Flow with Application to Investigations of Vascular Cell Remodeling

DOI:
10.3791/53224-v

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07:30 min

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November 03, 2015

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, ,
1Department of Mechanical Engineering,University of Colorado at Boulder, 2Product Development,Covidien, Ltd.

Chapters

  • 00:05Title
  • 01:04Silanization and Biomolecule Functionalization of Glass Slide
  • 03:25Determination of Pulsatility Index
  • 04:39Flow Chamber Assembly
  • 05:56Results: Cell Morphology and Protein Expression Changes with Flow Conditions
  • 06:48Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol replicates physiological or pathological blood flow in vitro to aid in determining cell response in disease pathologies. By introducing a pressure damping chamber downstream of a blood pump, blood flow across the vasculature can be recapitulated and imposed on a monolayer of vascular endothelium or a mimetic co-culture.

Tags

In Vitro ModelPhysiological Blood FlowPathological Blood FlowVascular Cell RemodelingVascular DiseaseFlow DynamicsPulsatility IndexEndothelial CellsSmooth Muscle CellsFibroblastsMechano-transductionCytokine ReleaseCo-cultureCardiac OutputArterial Wall CompliancePressure Damping

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