01:15Synthesis of Polystyrene-block-poly(N-isopropylacrylamide) by Two-step Reversible Addition-fragmentation Chain Transfer (RAFT) Radical Polymerization
02:42Preparation of Silanized Hydrophobic Modified Glass Substrates
03:49Preparation of Langmuir Films and Film-transferred Surface
06:59Culturing Cells and Optimizing Cell Adhesion and Detachment on the Langmuir Film Transferred Surface
09:32Results: Fabrication of Langmuir Film-transferred Surface and Evaluation of Cell Adhesion, Detachment, and Cell Sheet Recovery
Nanoscaled sea-island surfaces composed of thermoresponsive block copolymers were fabricated by the Langmuir-Schaefer method for controlling spontaneous cell adhesion and detachment. Both the preparation of the surface and the adhesion and detachment of cells on the surface were visualized.