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JoVE Journal
Developmental Biology

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In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells
 

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

Article DOI: 10.3791/55268-v 08:21 min March 16th, 2017
March 16th, 2017

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Summary

Here, we present a protocol to efficiently generate human trophoblastic cells from human pluripotent stem cells using bone morphogenic protein 4 and inhibitors of the Activin/Nodal pathways. This method is suitable for the efficient differentiation of human pluripotent stem cells and can generate large quantities of cells for genetic manipulation.

Tags

Human Pluripotent Stem Cells Trophoblastic Cells Differentiation Bone Morphogenic 4 Signaling Pathways Placental Formation Cell Function Assays Extracellular Matrix Tissue Culture Plates MEFCM BFGF Cellular Clamps
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