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Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples
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Cancer Research
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JoVE Journal Cancer Research
Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples

Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples

DOI:
10.3791/55589-v

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07:43 min

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July 29, 2017

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, ,
1Department of Pathology,Leiden University Medical Center, 2Department of Otorhinolaryngology and Head and Neck Surgery,Erasmus University Medical Center, 3Center for Gynecological Oncology Amsterdam,VU Medical Center

Chapters

  • 00:05Title
  • 01:04Antigen Retrieval
  • 02:16Tissue Staining and Analysis
  • 05:12Results: Representative Formalin-fixed, Paraffin-embedded Oropharyngeal Cancer Tissue Staining and Analysis
  • 06:42Conclusion

Summary

Automatic Translation

Automatic Translation

Multiparameter fluorescence immunohistochemistry can be used to assess the number, relative distribution, and localization of immune cell populations in the tumor microenvironment. This manuscript describes the use of this technique to analyze T-cell subpopulations in oropharyngeal cancer.

Tags

ImmunohistochemistryT-cell SubpopulationsOropharyngeal Squamous Cell CarcinomaFormalin-fixed Paraffin-embeddedMulticolorBiomarkersImmune CellsCancer TherapyPatient StratificationImmune Therapies

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