Journal
/
Developmental Biology
/
Precise Cellular Ablation Approach for Modeling Acute Kidney Injury in Developing Zebrafish
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Precise Cellular Ablation Approach for Modeling Acute Kidney Injury in Developing Zebrafish

Precise Cellular Ablation Approach for Modeling Acute Kidney Injury in Developing Zebrafish

DOI:
10.3791/55606-v

•

09:53 min

•

June 03, 2017

•

, , , , ,
1Department of Biomedical Sciences,NYITCOM, 2Department of Basic Sciences,NYITCOM-A-State

Chapters

  • 00:05Title
  • 01:05Maintaining Embryos and Mounting Zebrafish for Live-imaging
  • 02:59Laser Ablation
  • 06:53Results: Acute Kidney in Zebrafish by Precise Cellular Ablation
  • 08:42Conclusion

Summary

Automatic Translation

Automatic Translation

This work presents a new in vivo model of segmental kidney injury using kidney GFP transgenic zebrafish. The model allows for the induction of the targeted ablation of kidney epithelial cells to show the cellular mechanisms of nephron injury and repair.

Tags

Kidney AblationZebrafishAcute Kidney InjuryNephron InjuryKidney RegenerationSpatial Temporal ControlLive ImagingPhoto AblationConfocal MicroscopyAgarose Embedding

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Kidney Regeneration in Adult Zebrafish by Gentamicin Induced Injury

Mechanical Vessel Injury in Zebrafish Embryos

Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration

Technique to Target Microinjection to the Developing Xenopus Kidney

Generation of Parabiotic Zebrafish Embryos by Surgical Fusion of Developing Blastulae

Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos

Surgical Ablation Assay for Studying Eye Regeneration in Planarians

Histological Analyses of Acute Alcoholic Liver Injury in Zebrafish

Evaluation of Injury-induced Senescence and In Vivo Reprogramming in the Skeletal Muscle

Visualization of Cellular Electrical Activity in Zebrafish Early Embryos and Tumors

Read Article