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A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry
DOI:

08:46 min

July 12, 2017

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Chapters

  • 00:05Title
  • 01:10Testicular Tissue Single Cell Suspension Generation
  • 02:26Cell Staining and Fluorescence Activiated Cell Sorting (FACS)
  • 05:33Results: Representative Testicular Cell Subset Analyses
  • 07:17Conclusion

Summary

Automatic Translation

This work describes the standardization of a method to obtain purified germ cell populations from testicular tissue of different mammalian species. It is a straightforward protocol that combines mechanical testis dissociation, staining with Hoechst-33342 and propidium iodide, and FACS sorting, with wide applications in comparative studies of male reproductive biology.

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