Journal
/
Medicine
/
In Vivo Imaging of Cx3cr1gfp/gfp Reporter Mice with Spectral-domain Optical Coherence Tomography and Scanning Laser Ophthalmoscopy
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
In Vivo Imaging of Cx3cr1gfp/gfp Reporter Mice with Spectral-domain Optical Coherence Tomography and Scanning Laser Ophthalmoscopy

In Vivo Imaging of Cx3cr1gfp/gfp Reporter Mice with Spectral-domain Optical Coherence Tomography and Scanning Laser Ophthalmoscopy

DOI:
10.3791/55984-v

•

06:19 min

•

November 11, 2017

•

, , ,
1Department of Ophthalmology and Department of Clinical Research,Bern University Hospital and University of Bern

Chapters

  • 00:05Title
  • 00:43Spectral-Domain Optical Coherence Tomography (SD-OCT)
  • 02:43Auto Fluorescence Imaging
  • 03:43SD-OCT Image Manual Retinal Thickness Measurement
  • 04:44Results: Representative OCT Scans and AF Images of Cx3cr1gfp/gfp Mouse Retinas
  • 05:50Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes how high-resolution imaging techniques such as spectral domain optical coherence tomography and scanning laser ophthalmoscopy can be utilized in small rodents, using an ophthalmic imaging platform system, to obtain information on retinal thickness and microglial cell distribution, respectively.

Tags

In Vivo ImagingCx3cr1gfp/gfp Reporter MiceSpectral-domain Optical Coherence TomographyScanning Laser OphthalmoscopyRetinal ThicknessMicroglial Cell DistributionNon-invasiveCorneal Contact LensInfrared ImagingAutofluorescence Imaging

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy

Intracranial Implantation with Subsequent 3D In Vivo Bioluminescent Imaging of Murine Gliomas

Doppler Optical Coherence Tomography of Retinal Circulation

Segmentation and Measurement of Fat Volumes in Murine Obesity Models Using X-ray Computed Tomography

Generation and 3-Dimensional Quantitation of Arterial Lesions in Mice Using Optical Projection Tomography

In Vivo Optical Imaging of Brain Tumors and Arthritis Using Fluorescent SapC-DOPS Nanovesicles

Non-invasive Imaging and Analysis of Cerebral Ischemia in Living Rats Using Positron Emission Tomography with 18F-FDG

Combined In vivo Optical and µCT Imaging to Monitor Infection, Inflammation, and Bone Anatomy in an Orthopaedic Implant Infection in Mice

Assessment of Viability of Human Fat Injection into Nude Mice with Micro-Computed Tomography

Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy

Read Article