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A Filtration-based Method of Preparing High-quality Nuclei from Cross-linked Skeletal Muscle for Chromatin Immunoprecipitation
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
A Filtration-based Method of Preparing High-quality Nuclei from Cross-linked Skeletal Muscle for Chromatin Immunoprecipitation
DOI:

10:04 min

July 06, 2017

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Chapters

  • 00:05Title
  • 01:04Nuclei Isolation from Cross-linked Skeletal Muscle
  • 04:11Sonication
  • 05:14ChIP
  • 07:53Results: Purification of High-quality Nuclei from Cross-linked Skeletal Muscle Suitable for Chromatin Immunoprecipitation
  • 08:51Conclusion

Summary

Automatic Translation

We present a filtration-based protocol to isolate high-quality nuclei from cross-linked mouse skeletal muscle wherein we removed the need for ultracentrifugation, making it easily applicable. We show that chromatin prepared from the nuclei is suitable for chromatin immunoprecipitation and likely chromatin immunoprecipitation sequencing studies.

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