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Characterizing Cell Migration Within Three-dimensional In Vitro Wound Environments
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Bioengineering
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JoVE Journal Bioengineering
Characterizing Cell Migration Within Three-dimensional In Vitro Wound Environments

Characterizing Cell Migration Within Three-dimensional In Vitro Wound Environments

DOI:
10.3791/56099-v

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06:10 min

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August 16, 2017

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,
1Joint Department of Biomedical Engineering,North Carolina State University and The University of North Carolina – Chapel Hill, 2Comparative Medicine Institute,North Carolina State University

Chapters

  • 00:05Title
  • 00:44Cell and Spheroid Preparation
  • 02:183D Matrix Spheroid Embedding, Treatment, and Cell Migration and Proliferation Evaluation
  • 04:21Results: Representative Effects of Pro- and Anti-migratory Agents on In Vitro Fibriblast Migration
  • 05:13Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to evaluate the effect of pro- and anti-migratory factors on cell migration within a three-dimensional fibrin matrix.

Tags

Cell Migration3D In Vitro Wound EnvironmentFibrin ScaffoldWound HealingCell SpheroidsFibroblastsHanging Drop CultureFibrin Clot

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