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Journal / Genetics / Laser Microirradiation per lo Studio In Vivo…
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JoVE Journal Genetics
Laser Microirradiation per lo Studio In Vivo delle risposte cellulari al danno al DNA semplici e complesse
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Laser Microirradiation per lo Studio In Vivo delle risposte cellulari al danno al DNA semplici e complesse

Article DOI: 10.3791/56213-v • 10:44 min • January 31st, 2018
January 31st, 2018 •
*1, *2, 2, 2, 2, 2,3,4, 1
1Department of Biological Chemistry, School of Medicine, University of California, Irvine, 2Beckman Laser Institute and Medical Clinic, University of California, Irvine, 3Department of Developmental and Cell Biology, School of Biological Sciences, University of California, Irvine, 4Department of Biomedical Engineering and Surgery, University of California, Irvine
* These authors contributed equally

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L'obiettivo del presente protocollo è quello di descrivere come utilizzare laser microirradiation per indurre diversi tipi di danno del DNA, compreso le rotture del filo relativamente semplici e danno complesso, per studiare assieme di fattore DNA danni segnalazione e riparazione presso siti di danno in vivo .

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Genetica problema 131 microirradiation Laser laser in medicina danno del DNA risposta al danno al DNA riparazione del DNA la poli (ADP-ribosio) polimerasi (PARP) complesso danni rotture danni di base del filo
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