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Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea
DOI:

07:35 min

January 24, 2018

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Chapters

  • 00:05Title
  • 01:17Isolation of Primary Cells
  • 04:04Primary HCF Culture and 3D Construct Assembly
  • 05:00Cell Differentiation and Co-culture
  • 06:17Results: Transmission Electron Microscopy of the 3D Self-assembled Construct
  • 06:48Conclusion

Summary

Automatic Translation

This protocol describes a novel three-dimensional in vitro model, where corneal stromal cells and differentiated neuronal cells are cultured together to assist in the examination and understanding of the interactions of the two cell types.

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