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JoVE Journal Genetics
Generación de un gen interrumpido Streptococcus mutans cepa sin gen clonación
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Generación de un gen interrumpido Streptococcus mutans cepa sin gen clonación

Article DOI: 10.3791/56319-v • 12:06 min • October 23rd, 2017
October 23rd, 2017 •
1, 1, 1,2,3, 1
1Department of Translational Research, Tsurumi University School of Dental Medicine, 2Endowed Department of International Oral Health Science (affiliated with Department of Translational Research), Tsurumi University School of Dental Medicine, 3Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University

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Se describe un método fácil, rápido y relativamente barato para la generación de gene-disrupted Streptococcus mutans cepas; Esta técnica puede ser adaptada para la generación de gene-disrupted cepas de varias especies.

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Genética número 128 interrupción del Gene construcción de ADN reacción en cadena de polimerasa primer diseño gen marcador de resistencia a los antibióticos Streptococcus mutans electroporación recombinación homóloga
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