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Characterization of Cell Membrane Extensions and Studying Their Roles in Cancer Cell Adhesion Dynamics
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Characterization of Cell Membrane Extensions and Studying Their Roles in Cancer Cell Adhesion Dynamics

Characterization of Cell Membrane Extensions and Studying Their Roles in Cancer Cell Adhesion Dynamics

DOI:
10.3791/56560-v

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08:11 min

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March 26, 2018

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, , , ,
1Department of Surgery & Yale Endocrine Neoplasia Laboratory,Yale University School of Medicine, 2Yale New Haven Hospital

Chapters

  • 00:05Title
  • 01:10Imaging Cell Extensions
  • 03:55Adhesion Assay
  • 06:21Results: An In Vitro Quantitative Method to Characterize Cell Extensions Reveals the Role of Dickkopf-related Protein 3 (DKK3) in Cell Adhesion
  • 07:30Conclusion

Summary

Automatic Translation

Automatic Translation

This study demonstrates the utility and ease of quantitative cell membrane extension measurement and its correlation to adhesive capacity of cells. As a representative example, we show here that Dickkopf-related protein 3 (DKK3) promotes increased lobopodia formation and cell adhesiveness in adrenocortical carcinoma cells in vitro.

Tags

Cell Membrane ExtensionsCell AdhesionCancerCell BehaviorCell MotilityCell InvasionCell Extension RepertoireTumor Cell AdhesionTumor Cell InvasionCell Extension FormationIon Channel RegulationCell ImagingCell CountingCell FixationCell StainingMicroscopy

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