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Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth Activity
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth Activity
DOI:

08:52 min

March 30, 2018

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Chapters

  • 00:04Title
  • 01:06Culture Preparation of Dissociated Adult DRG Neuron
  • 03:41Co-culture of Primary Peritoneal Macrophages on A Cell Culture Insert
  • 05:47Neurite Outgrowth Assay with Collected Conditioned Medium
  • 07:05Results: Representative Results of Neurite Outgrowth Assay Using Conditioned Medium Obtained from Various Conditions
  • 07:55Conclusion

Summary

Automatic Translation

The current protocol presents experimental procedures to stimulate cultured macrophages to be endowed with capacity to release molecular factors that promote neurite outgrowth. Treatment of cAMP to the neuron-macrophage co-cultures induces the macrophages to produce conditioned medium that possesses strong neurite outgrowth activity.

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