/
/
Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth Activity
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:04Title
- 01:06Culture Preparation of Dissociated Adult DRG Neuron
- 03:41Co-culture of Primary Peritoneal Macrophages on A Cell Culture Insert
- 05:47Neurite Outgrowth Assay with Collected Conditioned Medium
- 07:05Results: Representative Results of Neurite Outgrowth Assay Using Conditioned Medium Obtained from Various Conditions
- 07:55Conclusion
The current protocol presents experimental procedures to stimulate cultured macrophages to be endowed with capacity to release molecular factors that promote neurite outgrowth. Treatment of cAMP to the neuron-macrophage co-cultures induces the macrophages to produce conditioned medium that possesses strong neurite outgrowth activity.
