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Extraction of Hemocytes from Drosophila melanogaster Larvae for Microbial Infection and Analysis
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Extraction of Hemocytes from Drosophila melanogaster Larvae for Microbial Infection and Analysis
DOI:

08:54 min

May 24, 2018

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Chapters

  • 00:05Title
  • 00:45Ex Vivo Infection
  • 01:48Hemolymph Extraction
  • 03:11In Vivo Infection
  • 04:08Hemolymph Extraction and Hemocyte Plating
  • 04:35Confocal Imaging
  • 05:443D Model Reconstruction
  • 06:58Results: Dynamic Visualization of Drosophila Hemocytes
  • 08:09Conclusion

Summary

Automatic Translation

This method demonstrates how to visualize pathogen invasion into insect cells with three-dimensional (3D) models. Hemocytes from Drosophila larvae were infected with viral or bacterial pathogens, either ex vivo or in vivo. Infected hemocytes were then fixed and stained for imaging with a confocal microscope and subsequent 3D cellular reconstruction.

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