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Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes
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Chapters
- 00:04Title
- 01:11Isolation of Tumor Associated Monocyte-derived DC
- 04:07Isolation of Monocyte-derived DC from Peripheral Blood
- 06:54Preparation of Tumor-IgG Immune Complexes Followed by Activation of MoDC with Tumor-IgG
- 08:58Results: Isolation and Analysis of the Activation of MoDC with the Tumor Immune Complex
- 11:02Conclusion
Monocyte-derived DC (MoDC) can sense minor amounts of danger-associated molecules and are therefore easily primed. We provide a detailed protocol for the isolation of MoDC from blood and tumors and their activation with immune complexes while highlighting key precautions that should be considered in order to avoid their premature activation.
