Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Environment / Specific and Accurate Detection of the Citrus…
JoVE Journal
Environment

A subscription to JoVE is required to view this content.

JoVE Journal Environment
Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples
Generating Homo- and Heterografts Between Watermelon and Bottle Gourd for the Study of Cold-responsive MicroRNAs
Journal (Environment)
Generating Homo- and Heterografts Between Watermelon and Bottle Gourd for the Study of Cold-responsive MicroRNAs
Sample Preparation Method of Scanning and Transmission Electron Microscope for the Appendages of Woodboring Beetle
Journal (Environment)
Sample Preparation Method of Scanning and Transmission Electron Microscope for the Appendages of Woodboring Beetle
 

Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples

Article DOI: 10.3791/57240-v • 09:23 min • June 29th, 2018
June 29th, 2018 •
*1,2, *1, 1,2, 1,2, 3, 2, 1
1Department of Biological Sciences, University of South Carolina, 2Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, 3Department of Instructions and Teacher Education, University of South Carolina
* These authors contributed equally

Chapters

Summary
Automatic Translation

Citrus Greening is a particularly destructive disease affecting citrus crops globally. Presented here is a simple method using PCR and genomic DNA extraction of citrus leaf tissue for the accurate and precise identification of the citrus greening pathogen, Candidatus liberibacter spp.

Tags

Citrus Greening Pathogen Detection Candidatus Liberibacter Spp. Conventional PCR Citrus Leaf Tissue Samples Citrus Farmers Citrus Greening Research Ian Palmer Graduate Student Efficient Detection Method Cost-effective Analysis Minimum Laboratory Experience And Equipment Leaf Tissue Preparation Liquid Nitrogen Chilling Grinding Leaf Tissue Green Powder Metal Spatula Micro-centrifuge Tube Nuclei Lysis Solution Incubation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter