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Immunology and Infection
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Generation of Large Numbers of Myeloid Progenitors and Dendritic Cell Precursors from Murine Bone Marrow Using a Novel Cell Sorting Strategy
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Immunology and Infection
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JoVE Journal Immunology and Infection
Generation of Large Numbers of Myeloid Progenitors and Dendritic Cell Precursors from Murine Bone Marrow Using a Novel Cell Sorting Strategy

Generation of Large Numbers of Myeloid Progenitors and Dendritic Cell Precursors from Murine Bone Marrow Using a Novel Cell Sorting Strategy

DOI:
10.3791/57365-v

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09:05 min

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August 10, 2018

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,
1Department of Biological Sciences,Auburn University

Chapters

  • 00:04Title
  • 00:40Collection of Murine Bone Marrow Cells
  • 03:57Staining Strategy
  • 05:53Set Gates Based on Control Samples
  • 06:22Collection of Isolated Populations
  • 07:20Results: Robust Myeloid Progenitors Using Protocol
  • 08:43Conclusion

Summary

Automatic Translation

Automatic Translation

Here we provide a method for identifying and isolating large numbers of GM-CSF driven myeloid cells using high speed cell sorting. Five distinct populations (Common myeloid progenitors, granulocyte/macrophage progenitors, monocytes, monocyte-derived macrophages, and monocyte-derived DCs) can be identified based on Ly6C and CD115 expression.

Tags

Myeloid ProgenitorsDendritic Cell PrecursorsBone MarrowCell SortingDevelopmental ImmunologyBone Marrow TransplantationHind LegsFemurTibiaMarrowCell IsolationCell CultureCell Preparation

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