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Generation of Human Nasal Epithelial Cell Spheroids for Individualized Cystic Fibrosis Transmembrane Conductance Regulator Study
JoVE Journal
Medicine
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JoVE Journal Medicine
Generation of Human Nasal Epithelial Cell Spheroids for Individualized Cystic Fibrosis Transmembrane Conductance Regulator Study
DOI:

08:00 min

April 11, 2018

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Chapters

  • 00:04Title
  • 00:41Passage Human Nasal Epithelial Cells
  • 02:45Seeding Cells for HNE Spheroid Cultures
  • 04:37Culture Differentiated and Mature HNE Spheroids
  • 05:27Results: Individualized CFTR Study Through the Generation of HNE Spheroids
  • 07:01Conclusion

Summary

Automatic Translation

Here we describe a method to generate three-dimensional spheroid cultures of human nasal epithelial cells. Spheroids are then stimulated to drive Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)-dependent ion and fluid secretion, quantifying the change in the spheroid luminal size as a proxy for CFTR function.

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