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A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo

DOI:
10.3791/57554-v

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11:17 min

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August 11, 2018

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, , , , , ,
1Department of Infectious Disease Immunology, Center for Vaccine Research,Statens Serum Institut, 2Department of Infectious Diseases,Hvidovre Hospital, 3Institute of Clinical Medicine, Faculty of Health and Medical Sciences,University of Copenhagen, 4Division of Infection and Immunity,University College London

Chapters

  • 00:00Title
  • 01:31Tuberculin Skin Test
  • 03:56Evaluation of Skin Reaction
  • 07:24Harvest of Blister Fluid
  • 08:35Results
  • 10:12Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we provide a demonstration of the suction blister cutaneous recall model. The model allows a simple access to study human in vivo adaptive immune responses, for instance in the context of vaccine development.

Tags

Suction BlisterT-cell Recall ResponseAdaptive ImmunityPPDTuberculosisBCG VaccineSkin TestIntradermal InjectionIn VivoAntigen-specific T-cellsCytokines

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