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Protrusion Force Microscopy: A Method to Quantify Forces Developed by Cell Protrusions
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JoVE Journal Biology
Protrusion Force Microscopy: A Method to Quantify Forces Developed by Cell Protrusions

Protrusion Force Microscopy: A Method to Quantify Forces Developed by Cell Protrusions

DOI:
10.3791/57636-v

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06:37 min

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June 16, 2018

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, , , , , , , ,
1Institut de Pharmacologie et Biologie Structurale,IPBS, Université de Toulouse, CNRS, UPS, 2METi, 3CNRS, LAAS, 4Univ de Toulouse, INSA

Chapters

  • 00:00Title
  • 00:45Preparation of Formvar-coated grids
  • 01:46Measurement of film thickness
  • 02:57Seeding cells on grids
  • 04:00Topography measurements of podosome-induced film deformations
  • 05:13Representative results
  • 06:18Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we detail the experimental techniques used to evaluate the protrusion forces that podosomes apply on a compliant film, from the preparation of the film to the automated analysis of topographical images.

Tags

Protrusion Force MicroscopyAtomic Force MicroscopyCell ProtrusionsFormvar FilmCell AdhesionPodosomesMechanical ModelFilm Thickness MeasurementContact Mode AFM

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