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Studerar ribonucleotid införlivande
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Studerar ribonucleotid införlivande: Strand-detektering av ribonukleotider i jäst genomet och mäta ribonucleotid-inducerad mutagenes

Article DOI: 10.3791/58020-v • 09:04 min • July 26th, 2018
July 26th, 2018 •
*1, *1, 1
1Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, NIH, DHHS
* These authors contributed equally

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Ribonukleotider är bland de vanligast förekommande icke-kanoniska nukleotider införlivas genomet under eukaryota nuclear DNA-replikation. Om inte korrekt bort, kan ribonukleotider orsaka DNA-skador och mutagenes. Här presenterar vi två experimentella metoder som används för att bedöma överflödet av ribonucleotid införlivande i DNA och dess mutagena effekter.

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Biologi fråga 137 ribonucleotid införlivande DNA-replikation RNase H2 alkaliska gel elektrofores fluktuation test mutagenes
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