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Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation
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Bioengineering
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JoVE Journal Bioengineering
Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation

Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation

DOI:
10.3791/58123-v

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06:28 min

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November 26, 2018

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, , , , , , , ,
1Regenerative Medicine Research,Texas Heart Institute, 2Lifegift Organ Donation Center, 3Biomedical Engineering,Texas A&M University

Chapters

  • 00:04Title
  • 00:38Organ Preparation and Decellularization
  • 03:38Results: Representative Whole Human Heart Decellularization Flow Rate Analysis
  • 05:18Conclusion

Summary

Automatic Translation

Automatic Translation

This method enables decellularization of a complex solid organ using a simple protocol based on osmotic shock and perfusion of ionic detergent with minimal organ matrix disruption. It comprises a novel decellularization technique for human hearts inside a pressurized pouch with real-time monitoring of flow dynamics and cellular debris outflow.

Tags

DecellularizationWhole Human HeartPressurized PouchInverted OrientationVascularized ScaffoldSeptal DefectVena CavaAortaPulmonary ArteryPulmonary VeinPerfusionPolyester PouchPBSHypertonic SolutionHypotonic SolutionSodium Dodecyl SulfateSDSPeracetic Acid

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