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Dynamic Adhesion Assay for the Functional Analysis of Anti-adhesion Therapies in Inflammatory Bowel Disease
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Dynamic Adhesion Assay for the Functional Analysis of Anti-adhesion Therapies in Inflammatory Bowel Disease
DOI:

08:27 min

September 20, 2018

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Chapters

  • 00:04Title
  • 00:45Capillary and Cell Preparation
  • 03:54Live Cell Imaging of Dynamic Adhesion and Evaluation
  • 06:52Results: Representative Human PBMC Adherence Assessment
  • 07:33Conclusion

Summary

Automatic Translation

Dynamic adhesion of immune cells to the vessel wall is a prerequisite for gut homing. Here, we present a protocol for a functional in vitro assay for the impact analysis of anti-integrin antibodies, chemokines or other factors on the dynamic cell adhesion of human cells using addressin-coated capillaries.

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