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Journal / Neuroscience / Biocytin Recovery and 3D Reconstructions of…
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Neuroscience

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JoVE Journal Neuroscience
Biocytin Recovery and 3D Reconstructions of Filled Hippocampal CA2 Interneurons
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Biocytin Recovery and 3D Reconstructions of Filled Hippocampal CA2 Interneurons

Article DOI: 10.3791/58592-v • 11:21 min • November 20th, 2018
November 20th, 2018 •
*1, *1, 1
1Department of Pharmacology, University College London
* These authors contributed equally

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Summary
Automatic Translation

The protocol outlined here describes the immunofluorescence analysis, biocytin recovery and high-quality reconstructions of hippocampal CA2 interneurons following the intracellular electrophysiological recordings in vitro, allowing neuronal characterization and ultimately fine neuronal anatomy to be studied.

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Biocytin Recovery 3D Reconstructions Hippocampal CA2 Interneurons Neuronal Subtypes Functional Map Cortical Circuitry Ultrastructure Preservation Dendritic Arbors Axonal Arbors Electrophysiological Recording Slice Transfer ACSF Filter Paper Fixative Solution Phosphate Buffer Tissue Trimming Gelatin Solution
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